The MethodologySection nests within the TestInformationSection and its text attribute consists of narrative describing methodology of the genetic test at stake. Where possible, use section entries to codify methods used in this genetic tests. Note that LOINC codes are being developed to capture methodology details for sequencing and gene chip tests and might be available in future releases.
Context
Parent nodes of template element with id 2.16.840.1.113883.10.20.20.1.9.2
Classification
CDA Section Level Template
Open/Closed
Open (other than defined elements are allowed)
Used by / Uses
Used by 1 transaction and 4 templates, Uses 0 templates
<section> <templateIdroot="2.16.840.1.113883.10.20.20.1.9.2"/><title>Methodology</title><text> <list> <item> <content> Exon 1 and the coding region of exon 2 of the connexin 26 (GJB2) gene are amplified using flanking primer sets. PCR products are sequenced using an ABI fluorescence automatic DNA sequencer. This test does not detect large deletions or mutations in non-coding regions that could affect gene expression. This assay is greater than 99.9% accurate in detecting mutations in the sequences analyzed. Polymerase chain reaction (PCR) analysis is performed to detect the presence or absence of a deletion spanning the GJB6-D13S1830 region of chromosome 13. </content></item></list></text></section>